Protocols

To support scientists in their experiments with our technology, React4life has prepared documents explaining how to use MIVO®. Download our protocols for free.
In addition, we can support scientists by also providing a MIVO® Video Tutorial. Request it with an email to info@react4life.com

MIVO® Absorption assay

By assessing compound permeability in the early stages of drug discovery, MIVO® device combined with tissues or artificial membrane provides a reliable platform for fluid-dynamic in vitro permeability studies that predict the in vivo absorption for your compounds.

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MIVO® Cytotoxicity assay

The following protocol outlines the steps for a cytotoxicity assay to be performed on with MIVO® device. Different metabolic assays such as the AlamarBlue™, the MTT or the Presto BlueTM Cell Viability Reagents can be purchased and used for the evaluation of cell cytotoxicity within MIVO.

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MIVO® Immunostaining assays

The following protocol outlines the steps for a generic immunostaining assay and it is referred to the use of cellularized Transwell supports or organoids cultured within the MIVO chamber Using MIVO® devices it is possible to perform immunostaining assays employing a microscopy as output detection.

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MIVO® Live/Dead assays

The following protocol outlines the procedure to perform the live/dead assay using MIVO® devices and the PromoKine’s Cell-Mediated Live/Dead Cell Staining Kit I for the evaluation of cell viability in vitro.
MIVO devices are compatible with other Live/Dead Assays which involve fluorescence quantification of the output.

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MIVO® Proliferation assay

The following protocol outlines the steps for a proliferation assay to be performed on MIVO® device. Different metabolic assays such as the AlamarBlue™, the MTT or the Presto BlueTM Cell Viability Reagents can be purchased and used for the evaluation of cell proliferation within MIVO®.

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