The Problem

Human biology is difficult to replicate in the lab.

Current research models, both in vitro and animal ones, are limited in their ability to mimic the extremely complex process of human physiology.
The result is that both of them too often fail to translate into treatments for humans.

In Vitro Models

Are Human
but not Systemic

Animal Models

Are Systemic
but not Human

The need

We all need humanized reliable models

There are more than 200 different cell types in the human body.

Although model simplification is still very common, it ends up in reductive models that cannot catch the real complexity of biological interactions, losing significant cell responses. MIVO platform supports both 3D culture and co-cultures for better resempling the in vitro human biology.
In particular, 3D cell culture show improvements in studies targeted towards morphology, cell number monitoring, proliferation, response to stimuli, differentiation, drug metabolism, and protein synthesis. All of this is made possible by 3D cultures' capability to model a cell in vivo while cultured in vitro, cells can grow and interact with the surrounding extracellular framework in the three dimensions.

3D cultures are more physiologically relevant and predictive

Three-dimensional cell culture systems are gaining increasing interest in drug discovery and tissue engineering due to their evident advantages in providing more physiologically relevant information and more predictive data for in vivo tests.
Interaction among different types of cells is more realistic in 3D cultures; moreover, the molecules (drugs and nutrients) diffuse within 3D cell culture in a more predictive way than the too simple 2D culture.

Each of our human districts has its own flow rate.

Fluid-dynamic conditions have a significant effect on cells survival, proliferation, migration and differentiation. Also, the level shear stress induced by the fluid flow and experienced by the cells is crucial for achieving more predictive results. The complete control of these parameters is critical to recapitulate the physiological conditions.

Multi-organ models can recapitulate biological complexity.

The crosstalk among organs is strongly important in research areas like drug safety and efficacy testing or immuno-oncology, where different orga-organ interactions is essential. Multi-organ models can give detailed information on both pharmacokinetics and biodistribution, adding biological complexity and mimiking physio-pathological conditions.

Our Solution
MULTI IN VITRO ORGAN DEVICE

Reliable

Perform realistic, controlled and reproducible tests in a 3D human and dynamic environment in vitro.

Quick & Easy

Disposable chambers assembled in 2min.

Customizable

Keep your cells and tissues and easily turn your protocol assays into the MIVO® device.

User-friendly

Neither technological skills nor additional equipment investments are required.

Added value

Find out MIVO® applications and make it your new standard

3 AREAS OF APPLICATION

IMPROVE TISSUES CONDITIONS AND PROLONG CELLS LIFETIME

Ex-vivo cultures

Patient biopsies can be exploited for drug testing and personalized medicine, as long as their culture conditions are optimized to resemble the physiological environment and support cells survival ex vivo.

Controlled Dynamic flow

Controlled dynamic models mimicking the physiological flows recreate tissue perfusion and shear stresses stimuli, affecting cell behavior toward a more relevant and predictive condition.

ACCELERATE DRUG TESTING ON 3D HUMAN TISSUES WITHOUT INVOLVING ANIMAL USE

Biological barriers

The dynamic culture of biological barriers (i.e., skin, intestine, lung) allows studies regarding exchanges/retention of molecules across two physiological compartments.

Molecules diffusion

3D dynamic cultures better recapitulate interstitial diffusion processes, opening new ways for molecule tracing and distribution (eg. nanomedicine).

Multi-organ

Dynamic multi-organ connections allow to better emulate complex human physiological and pathological processes, as well as to perform pharmacokinetic studies (i.e. ADME).

3Rs principle

“Replace, Reduce, Refine” is the principle governing the responsible use of animals in research, raising the urgent need for reliable alternatives in vitro.

ACHIEVE RELIABLE HUMAN DISEASE MODELS FOR BASIC RESEARCH

3D cell cultures/Organoids

Simple or complex 3D human cells cultures better recapitulate healthy or diseased tissue architectures, dramatically affecting cell-to-cell and cell-to-matrix interactions.

Cell cocultures/Cell crosstalk

Cell-to-cell interactions studies represent the basis for the understanding of physiological and pathological processes at the molecular, cellular, tissue level.

Humanized in vitro modelling

Reliable tools for physiologically relevant human modelling represent the key to overcome experimental species-specific gaps, speeding up pharmacological research.

Cell infiltration

Complex 3D human dynamic cultures are powerful tools to study the infiltrating potential of different cell types (i.e., immune cells, endothelial cells, cancer cells).

Case History

There's no limit to the results you can achieve with MIVO®.
You can set the most extensive range of experiments and immediately realize your progress.
Check out some case history and learn about tremendous improvements on results are possible thanks to the fluid flow, the 3d culture and the flexibility of our device.

Get your hands-on experience, check our workshops
Case study

Immune cells

Case study

Liver

Case study

Gut

Case study

Skin

Case study

Cardio

Case study

Ovarian

Case study

Breast

Case study

Neuroblastoma

Case Study: Breast

Breast cancer is the most common cancer in women across most ethnic groups and one of the leading causes of cancer-related deaths worldwide. Mortality is mainly associated with the development of metastases, that is the spreading of tumour cells from the primary site to other parts of the body. MIVO® platform allows to culture fully-humanized breast cancer tissues, eventually in physical contact with a vascular barrier, to monitor and measure the tumour cells migration, spreading, intravasation (i.e. passage across the vascular barrier) and infiltration towards the metastatic site. The circulating tumour cells (CTC) survival under the fluid flow-induced shear stress can be also investigated within MIVO®.

Case Study: Neuroblastoma

3D tumour model with the proper molecular features that more closely resemble the immunophenotypic variants can be recapitulated in vitro within the MIVO® platform to obtain a clinical-relevant cell culture platform where testing the efficacy of personalized therapeutic approaches and optimize the current and innovative immune-based therapies in a very systematic and reliable way.

The adoption of the MIVO® device is for a functional immune-oncology screening platform but also for cell-based therapies that could be infused into the platform for testing their journey and activity toward tumour cells, like neuroblastoma cancer cells.

Case Study: Ovarian

3D in vitro cancer models are becoming day-by-day more important as promising alternatives to animal tests for establishing the efficacy of anticancer treatments in vitro. The culture of 3D fully-humanized tumour tissues, such as ovarian tumour models, within an in vivo like fluid-dynamic environment within MIVO®, allows recapitulating the capillary blood flow that feeds the tumour and to properly diffuse drug molecules within the 3D matrix, for more predictive results than static in vitro model.

To know more, read our publication.

Case Study: Cardio

Adverse side effects to the heart have become one of the most common causes for preclinical project closures since preclinical models do not fully recapitulate human in vivo dynamics and select the wrong drug. MIVO® organs-on-a-chip technologies allow mimicking the dynamic conditions of the cardiac niche, in terms of flow velocities and shear stress. Both cardiac cell lines, primary cells and cardiac organoids can be hosted within MIVO® chambers under flow conditions. Both tangential and perfusing flow can be properly set up.

These dynamic cardiac models provide a challenging asset for safer and more effective drugs and personalized therapies.

Case Study: Skin

Dermal absorption assays are employed to assess the absorption kinetics of different cosmetic formulations, medical devices and drugs. Depending on the application field (pharmaceutics, cosmetics, toxicology), dermal absorption assays aim to demonstrate the successful delivery of active ingredients, to predict the risks related to the exposure to potentially toxic molecules or to prove the absence of absorption of specific substances classes.

MIVO® is a diffusive chamber compliant with the OECD TG 428 guideline for dermal absorption purposes.

This study highlighted in MIVO® allows the management of artificial skin membranes, human reconstructed tissues or skin biopsies and the easy and fast sampling system allows obtaining dermal absorption kinetics with high automatization and reproducibility.

Case Study: Gut

Human gut physiologically relevant models are requested for more predictive permeation and pharmacokinetic assays.

Through a precise set-up of the physiological fluid flow conditions of the human gut, and a fast and easy multiple sampling of the media, the MIVO® platform can be utilized to evaluate the passage and biodistribution of molecules orally administered, with high predictability and reproducibility. Both CaCO-2 cells monolayers, biomimetic membranes and 3D human intestine tissues can be selected as a gut model and cultured within MIVO chambers, overpassing the limits of the current static in vitro models about the relevant barrier function and molecules diffusion kinetics.

Case Study: Liver

The liver is the organ where each drug and molecule is processed; hence, understanding potential adverse effects are a pivotal part when studying metabolism and toxicology into a drug development process.

Researchers can use our organ on chip MIVO® platform to shape their liver disease models, identify toxicity markers and investigate drug metabolism in vitro.

Tissues continuously undergo the physiological and controlled fluidic flow, that improves mass transports for the proper in vivo like liver tissue feeding and perfusion. Moreover, the MIVO® multi-organ platform supports the fluidic connection of other organs (like gut and kidney) to make it even more predictive on ADME and pharmacokinetic assays.

Case Study: Immune cells

The development of immunotherapeutic drugs is strictly dependent on the interactions between human cancer and immune cells in a 3D and dynamic environment. Preclinical safety and efficacy assessment of immunotherapies are currently carried out through too simplistic in vitro 2D cell cultures and in vivo xenografts where complete immune compatibility, remains an important challenge.

The combination of tumour models (either 2D or 3D) and fluid-dynamic cells culture within MIVO® improves the relevance of in vitro pre-clinical systems by reproducing the dynamic complex interactions between tumour cells and immune effectors transported in situ via the systemic circulation.