FAQ2020-11-04T14:41:42+00:00

FAQ

If you would like to learn more about our products, services and others, or If you are a current customer and cannot find the answer to your question below please, contact us.

R1001J React4life Peristaltic Pump2020-11-04T14:48:23+00:00

Q – What are the function of the roller and what does the number of rollers mean for the function of the device?
A – The rollers impact on smoothness of the flow: higher the number of the rollers smoother is the flowand lower is the max flow rate.

Q – Do we need 1 channel for each MIVO device?
A – Each MIVO requires one channel; channels can manage independent experiments since MIVO based circuits are indipendent.

Q – Do we need a reservoir?
A – It Is your choice: most users typically adopt MIVO in close circuit making a continuous recirculation of the media (about 4ml) without using ant reservoir. Nevertheless, if you prefer a fresh media circulation, you may use MIVO connected with fresh media reservoir (in input) and waste reservoir (in output).

Q – Is it possible to set the inner diameter of the pump tubing as input of the pump and receive in output the actual flow rate?
A – According to the inner diameter of the pump tube you use, the flow rate will change. We may support customers to set up the proper flow rate (setting the proper rpm in the pump) according to your tubes, in order to have the required velocity in the cellular chamber.

Q – How does the user calibrate the pump? Do you need additional equipment or some flow rate sensors to do this?
A – You don’t need to calibrate the pump, we have already done it for you and the relation between rpm and velocities at different tube diameters is already described in the pump manual.

Q – Is it possible for you to send us the manual of the pump in PDF format?
A – The instruction manual is provided with the Pump

Frequently Asked Questions2020-11-04T14:52:29+00:00

ABOUT MIVO® DEVICE PRODUCTS

Q – What is included in the MIVO® Starting Kit?
A – The starting kit contains 3 MIVO® chambers, 3 tips and 1 plate.

Q – What is included in the single-flow box?
A – The bulk box contains 12 MIVO chambers, 12 tips.

Q – Does the bulk box contains the plate to hold the receivers?
A – No, it doesn’t. It has 12 disposable chambers, since you may use the plate (reusable) of the starting kit. Of course, If you will use directly the bulk box, we may provide a plate as well.

Q – How many chambers host the plate?
A – Three.

Q – Which are the differences between the single-flow box and the double-flow box?
A – The single flow box contains 12 closed lips, like those of the Petri dish, while the double flow box contains 12 tubes lips for a second circulation.

Q – What is included in the double-flow box?
A – The bulk box contains 12 MIVO chambers, 12 tips with their tubes.

ABOUT MIVO® TECHNOLOGY

Q – How does MIVO work?
A – Together with your order, you will receive a training video explaining the use of the MIVO, the loading of the culture media, its sampling, etc.

Q – How is sterility maintained?
A – the MIVO is already sterile and sold in single use blisters

Q – Which membrane is there in the MIVO chamber?
A – MIVO is typically sold without any membrane, since the end-user may decide to use the membrane preferred among Corning transwell, Falcon transwell, 3D tissue inserts, etc. In case it is appreciated, we may insert in the quotation also the membranes to combine in one order everything.

Q – What types of commercial inserts are compatible with the MIVO chambers?
A – To our knowledge Alcyomics, Cellsystem, Corning, Falcon, Mattek, Millipore.

Q – Is the trans-well a clickable insert, or a fixed insert?
A – MIVO chamber can be used with all type of inserts. Also the pore size and material of the transwell is a choice of the end-user. The insert is just placed inside the chamber without any external fixing. At the end of the experiment, the transwell can be easily recovered for further analysis.

Q – Which is the size of the hosted inserts?
A – the 24-well plate size inserts.

Q – What is the material of the MIVO and tubing?
A – It is a secret information, but we can say that they are not PDMS, and that they are FDA and USB class VI compliant, and DEHP free. We have tested different molecules absortions with great results of absorption (i.s. no absorption).

Q – Does the lid allow sterile gas exchange?
A – Yes, the MIVO lip works similarly to the lid of a Petri dish: the gas exchange happens in a sterile way.

 

MIVO IN YOUR LAB

Q – What other types of materials are required (e.g. tubing, pumps)?
A – MIVO chamber is already connected with tubes. The user has to connect the MIVO device with the own peristaltic pump tubes ending with male/female luer-locks. The inner diameter of the peristaltic pump tube depends on the flow rate required, but our team will give support to setup the pump. Regarding the pump, if the experiment is long-term experiment that requires to place MIVO within the incubator, you have to work with either peristaltic pump supporting humidity and 37°C environment or syringe pumps.

Q – Shall I culture organoids within MIVO?
A – Yes, spheroids, organoids or 3D scaffolds having different size and shape can be hosted within the MIVO under dynamic flow perfusion 

Q – How do we keep the chambers upright in an incubator?
A – In the incubator, you will have the MIVO chambers properly fixed on their plate and fluidically connected with the pump thanks to the tubes stopper of the plate.

Q – How easy is it to insert our own membrane within the MIVO?
A – When you will start the experiment, you will open the sterile blister containing the MIVO chamber and then place your membrane (transwell ,3D reconstructed tissue, or sterile membrane) directly within the MIVO chamber with a tweezer; the inner chamber has proper steps so that the membrane remains flat and stable; then you will open the blister containing the sterile tip and you will close the chamber.

Q – How can we sterilize the MIVO after the membrane insertion?
A – You don’t have to sterilize anything since you will place your sterile membrane directly within the sterile MIVO chamber under the biological hood.

Q – Shall I culture organoids within MIVO?
A – Yes, spheroids, organoids or 3D scaffolds having different size and shape can be hosted within the MIVO under dynamic flow perfusion.

Q – How do we keep the chambers upright in an incubator?
A – In the incubator, you will have the MIVO chambers properly fixed on their plate and fluidically connected with the pump thanks to the tubes stopper of the plate.

Q – Can you provide details of how we can incorporate an electro-spun membrane?
A – We have realized an add-on of the MIVO for placing a membrane within the chamber and avoid any type of fluid leakage. These add-ons can be used for biopsies, commercial membranes like Merck StratM or electro-spun membranes.

Q – Can I place the MIVO into the incubator?
A – Yes. the MIVO chambers can be easily move into the incubator, since they are blocked on their tray. The tubes are also blocked on the tray with stoppers.

Q – Is it optically transparent and usable under optical microscope?
A – The material of the MIVO is optically transparent and the lower window thickness allows to observe the cells cultured within MIVO under standard inverted microscope.

Q – Does the starting box contain protocols for seeding cells, and information on flow?
A – You may download a technical brochure of the product, where some informations are already available. Moreover, some experimental protocols are available on our website. Use also our support mail for receiving the R&D team remote support.

About MIVO® device product2020-11-04T14:49:29+00:00

Q – What is included in the MIVO® Starting Kit?
A – The starting kit contains 3 MIVO® chambers, 3 tips and 1 plate

Q – What is included in the BULK box?
A – The bulk box contains 12 MIVO chambers, 12 tip

Q – The bulk box has one plate (for three units) and 12 disposable receivers?
A – It has only 12 disposable receivers, since you may use the plate (reusable) of the starting kit. Of course, If you will use directly the bulk bow, we may provide a plate as well.

Q – The bulk does not contain a plate to hold the receivers?
A – The bulk typically doesn’t contain the plate for those who have already bought the starting kit

Q – Can you make a plate with 4 receivers?
A – At the moment no

Q – MIVO is ‘disposable’, but does that mean none of the elements can be reused?
A – Only the pump tubes are reusable

Q – From the brochure it’s hard to see how MIVO works in detail
A – With your order you will receive a training video explaing the use of the MIVO, the loading of the culture media, its sampling, etc.

Q – The upper chamber is covered wither with a lid, or a circulation unit, sketchily indicated in the figure. Are both part of the kit?
A – Both: the starting kit contains the two different versions of the lid: the closed lid, and the li having two tubes, for a second circulation circuit. According to your need, you may then select either the bulk box with 12 closed lips or the box with the tubing lids.

Q – How is sterility maintained?
A – the MIVO is already sterile and sold in single use blisters