MIVO^®-Based Cancer Model Resembling Systemic Drug Administration for Efficacy Assay

This application note presents a 3D ovarian cancer model developed on the MIVO® Single Flow platform, using SKOV-3 cells encapsulated in FlowGel® to mimic the tumor extracellular matrix. By applying physiological flow conditions, the study simulates systemic cisplatin administration and compares drug efficacy outcomes against traditional static cultures and in vivo xenograft data—demonstrating improved predictivity for preclinical oncology screening.

In this study, a 3D ovarian cancer model was established using SKOV-3 cells encapsulated in 3D FlowGel, a biomimetic hydrogel resembling the tumor extracellular matrix. The 3D model was cultured within the MIVO® Single Flow organ-on-chip under physiological flow conditions. This setup was used to simulate systemic cisplatin administration.
Drug efficacy was assessed over time within the MIVO® system and compared to both conventional static in vitro culture and an in vivo xenograft mouse model. Cell viability and proliferation were evaluated through Alamar Blue, live/dead staining, and immunostaining.
The results demonstrate that the dynamic, in vivo-like microenvironment recreated in the MIVO® platform enables a more physiologically relevant 3D tumor model and provides improved predictive values for in vivo drug responses compared to traditional static culture systems.