Organ-on-chip based in vitro approaches for co-culturing 3D human cancer tissues and immune cells for more predictive drug testing and human disease modeling

The human disease modeling for basic research and drug testing purpose siscurrently carried out through 2D cell culture in static conditions, and in vivo xenografts or genetically engineered animal models, but predictability, reliability, and complete immune compatibility remain important challenges. Forthisaim, novel3D, fully humanized in vitro cancer tissue models have been recently optimized by adopting emerging technologies such as microphysiological systems (MPS) and 3D cell laden hydrogels. In particular, a novel Multi-In Vitro Organ (MIVO) MPS platform has been recently adopted to culture 3D clinically relevant size cancer tissues under proper physiological culture conditions to investigate the efficacy of anticancer treatments.

• Conference: CICON 2023, Milan, Italy
• Authors: E. Palamà¹, M. Aiello¹, S. Scaglione¹