3D cancer matrix-based models and Multi-Organ-on-Chip Platforms to investigate in vitro tumour cells invasion, survival under fluid flow and infiltration in metastatic target

Traditionally, human cancer modeling for both basic research and drug testing has heavily leaned on 2D cell cultures in static conditions, as well as in vivo xenografts or genetically modified animal models. However, these methodologies present challenges in terms of predictability, reliability, and immune compatibility. Critical questions remain about the metastatic journey of these cells from primary tumor to bone and the oftendiscrepant drug responses between clinical and preclinical studies. Current models inadequately capture this complexity, highlighting the necessity for advanced in vitro systems that more accurately reflect the metastatic process.

• • • Conference: EACR 2024, Rotterdam, Netherlands
    • Authors: E. Palama¹, M. Aiello¹, S. Scaglione¹