Cancer stem cells (CSCs) drive tumor progression, metastasis, and therapy resistance, making their reliable maintenance in vitro essential for the development of physiologically relevant 3D cancer models. However, conventional tumorsphere workflows are often limited by inconsistent sphere formation, variability in starting material, and the formation of heterogeneous clumps.

In this joint webinar, PromoCell introduces a streamlined, matrix-free workflow designed to support CSC-driven growth across multiple cancer types. Built around the 3D Tumorsphere Medium XF, this approach enables the formation of uniform, robust tumorspheres without the need for additional tools or extracellular matrices. We’ll share data demonstrating improved tumorsphere formation efficiency and successful long-term expansion of patient-derived tumor cells while preserving key tumor characteristics.

Building on this foundation, the MIVO^® cancer-on-chip platform will be presented as the next step in advancing these models from static to dynamic environments. We will demonstrate how integrating these robust spheroids into the MIVO^® technology allows for the recapitulation of key biophysical cues of the tumor microenvironment, such as interstitial fluid flow and shear stress. This integration enables a highly predictive platform for studying complex biological processes, including tumor cell dissemination, invasive patterns, and drug efficacy, bridging the gap between conventional in vitro assays and in vivo models.