Anti-itching lenitive activity

Pruritus, or itching, is a complex and multifaceted sensation, often driven by key inflammatory mediators. This assay provides a validated and reliable method for assessing the safety and efficacy of anti-pruritic formulations, assessing their ability to modulate inflammatory pathways and alleviate discomfort.

Purpose of the Test

The MIVO-based anti-itch test uses the Skin-on-Chip platform, to replicate human skin structure and function and precisely evaluate soothing efficacy of testing formulations, by measuring their impact on LTB4 production, a critical driver of inflammation and itching.

The MIVO® Advantage: A Superior Predictive Model

The MIVO® organ-on-chip technology revolutionizes traditional 3D models by introducing dynamic fluid flow (Pulsoni et al. 2022), creating a highly realistic simulation of physiological conditions. This innovative platform enables precise biomarker analysis, such as LTB4, while capturing complex cell interactions. MIVO® offers a predictive and reliable tool for evaluating anti-inflammatory and soothing effects with unparalleled accuracy.

Our Unique Value Proposition

Realistic, Humanized Model: The MIVO® platform integrates a 3D RhE with dynamic fluid flow, offering an exceptionally realistic simulation of skin’s response to pruritus.
Dynamic Fluid Flow for Enhanced Accuracy: Moving beyond static models, the integration of dynamic fluid flow enhances the interaction between skin cells, immune responses, and sensory pathways, delivering deeper, more accurate insights into the effectiveness of treatments aimed at soothing inflammation and relieving itching.
Comprehensive Evaluation: With a multiparametric approach, including precise biomarker quantification such as LTB4, our test provides a comprehensive evaluation of anti-pruritic formulations, measuring both inflammatory responses and the soothing effects of potential treatments.
Broad Applicability: Ideal for testing a wide range of anti-itch products, from pharmaceutical drugs to natural compounds and supplements, this assay offers a flexible and powerful solution for ensuring the efficacy and safety of pruritus-relieving treatments across multiple sectors.

Anti-itching Assay

In summary

Anti-itching lenitive activity summary
leverage our millifluidic test with mivo

READOUTS

  • Epithelial barrier integrity (TEER measurement) 
  • Histology
  • Inflammation (i.e. LTB4)
shooting Anti-itching lenitive activity

Soothing effect is monitored by
evaluating the expression of protective
markers.

teer Anti-itching lenitive activity

TEER measurement is performed before
and after exposure to itching stress, as
indirect measurement of barrier integrity.

Inflammation Anti-itching lenitive activity

The ability of anti-itching formulation to ameliorate the inflammation induced by itching stimuli is assessed by
quantification of pro-inflammatory cytokines (i.e. LTB4) through ELISA assay.

Get a Quote

We are here for you

Whether you’re looking for a quote or want to connect with us, fill out the form below with your details, and we will be in touch shortly.

All fields are required, take your time.