The oral mucosa has a thin epithelium and a rich vascularization that facilitate the absorption of xenobiotics, whose chemical-physical properties influence their permeation, following oral administration. The assessment of the permeability of a substance and the estimation of its absorption help to identify potential candidates, such as drugs or bioactive functional compounds with nutritional or physiological effect.

Today, the number of in vitro assays able to mimic the real physiological conditions present in vivo remains limited and therefore the challenge is to develop advanced in vitro models, for which the key parameters such as permeability of substances through membrane barriers and their integrity, can be easily controlled and monitored with a reduction in experimental times and costs.

In this work we propose a new in vitro device (MIVO®) that allows to cultivate an oral epithelial tissue model under physiological flow conditions, in order to better predict the response in vivo, for an absorption study of S-Adenosyl-L- Methionine (SAM-e), a donor molecule of methyl groups in the synthesis of neurotransmitters, contained in an innovative orobuccal formulation.



Daniela Buonocore obtained her Master degree of Science in Molecular Biology and Genetics and then her PhD degree in Biomedical Sciences, together with two Post-graduate Masters of II level in Human Nutrition and Regulatory Affairs. Since 2008, she has been working as young and senior researcher in the field of Pharmacology – Pharmacognosy, Biochemistry and Human Nutrition at the University of Pavia, in particular, focusing the studies on the pharmacological and functional aspects of nutraceutical supplements. She is Author of 41 scientific papers in peer-reviewed indexed international journals, including 2 guidelines and 2 contributes in book. She is Principal Investigator and coordinator of scientific task forces in national projects and grants. She is Co-founder of bioRESTART, academic Spin-off of the University of Pavia and Innovative Startup part of EIT Food by the European Union.

Federico Pialorsi obtained his Degree in Pharmacy in 2007.
Since 2008, he has been working for Truffini & Reggè Farmaceutici, where he holds the position of Marketing Manager with responsibility for product development and business strategy.

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