React4Life cancer experts developed a breakthrough fluidic platform for culturing tumor models and testing the migration and intravasation capacity of tumor cells in vitro.
This MIVO-based assay allows to cultivate tumor models in close contact with epithelial tissues with the function of vascular wall. The capillary blood flow below the epithelial barrier resembles the path where migrated tumor cells become circulating tumor cells, surviving to shear stresses inducted by the flow. This innovative assay allows growing 3D cancer models for immediate, easy to perform and affordable in vitro anti-metastasis drug testing.
Key passages of this assay are:
- Test in physiologically relevant fluid dynamic conditions the proliferation and activity of 3D matrix-based cancer cell culture
- Profile and quantify the cancer cell intravasation after their migration out the tumor model composed of single or cocultured cells