React4life has implemented a protocol to carry out absorption tests on test molecules by recreating the physiological environment in vitro.
Absorption critically determines the bioavailability of the compound. In order for a compound to reach a tissue, it usually needs to be included in the bloodstream – often through mucous surfaces such as the digestive tract (intestinal absorption) – before it is absorbed by the target cells. Factors such as poor solubility of the compounds, gastric emptying time, intestinal transit time, chemical instability in the stomach, and inability to permeate the intestinal wall allow to reduce the degree of absorption of a medical device after oral administration.
The innovative solution proposed by React4life, for the in vitro absorption test, involves the use of the patented technology MIVO which is compatible with most epithelial tissues (e.g. skin, vaginal wall, oral mucosa) commercially available or recreated in the laboratory through cell cultures. By connecting the MIVO with a peristaltic pump or syringe, it is possible to reproduce in vitro the systemic circulation that feeds the tissue and that influences the absorption kinetics that characterizes the real condition in vivo.